產(chǎn)品詳情介紹
本產(chǎn)品包含TransStart? FastPfu DNA Polymerase、dNTPs和優(yōu)化的反應(yīng)緩沖液，濃度為2×，擴增效率強，擴增速度快，具有高保真性、高特異性。DNA擴增時，只需加入模板、引物和水，使SuperMix溶液的濃度為1×即可進行反應(yīng)。擴增產(chǎn)物為平端，可直接克隆于pEASY?-Blunt系列載體中。2×TransStart? FastPfu PCRSuperMix (+dye)擴增產(chǎn)物可直接點樣電泳，如用于克隆，需純化去掉染料。其PCR產(chǎn)物不適用于聚丙烯酰胺凝膠電泳。
? 減少PCR擴增操作時間。
? 避免因多步操作帶來的污染。
? 保真性是EasyTaq? DNA Polymerase的54倍。
? 基因組DNA片段的擴增(≤15 kb)。
? Plasmid DNA片段擴增(≤20 kb)。
1 Song R, Guo P, Ren X, et al. A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer[J]. Molecular Cancer, 2023.(IF 37.30)
2 Jin S, Lin Q, Gao Q, et al. Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)[J]. Nature Protocols, 2022.(IF 17.02)
3 Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)
4 Wang Y, Wang Z, Chen Y, et al. A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii to understand the H2O2-sensing mechanism of OxyR[J]. Cell Chemical Biology, 2019.(IF 6.76)
