產(chǎn)品詳情介紹
本試劑盒適用于從細(xì)胞和組織中提取總RNA，用TransZol Up裂解樣品，加入RNA Extraction Agent后，溶液分為無色水相和粉紅色有機(jī)相，RNA在水相中；用硅膠膜離心柱特異吸附水相中的RNA，與其它總RNA提取方法相比，既具有TransZol Up裂解能力強(qiáng)、提取量高，應(yīng)用范圍廣的優(yōu)點(diǎn)，又具有離心柱提取RNA純度高的優(yōu)點(diǎn)。
RNA提取效率高
使用TransGen ER501產(chǎn)品，分別以REA和氯仿作為抽提試劑，提取等量的Hela細(xì)胞的RNA，瓊脂糖凝膠電泳分析提取效果。結(jié)果表明， TransGen 產(chǎn)品RNA提取效率高，REA與氯仿提取效果相當(dāng)。
對下游實(shí)驗(yàn)無擴(kuò)增抑制
使用TransGen ER501產(chǎn)品，分別以REA和氯仿作為抽提試劑，提取Hela細(xì)胞的RNA，用TransGen一步法RT-PCR產(chǎn)品 (AT411)分析擴(kuò)增效果。結(jié)果表明，使用REA作為抽提試劑提取的RNA對下游PCR實(shí)驗(yàn)無擴(kuò)增抑制。
使用TransGen ER501產(chǎn)品，分別以氯仿和REA作為抽提試劑，提取Hela細(xì)胞、煙草、小鼠組織的RNA，分別用TransGen一步法RT-qPCR產(chǎn)品(AQ211)和兩步法RT-qPCR產(chǎn)品 (AUQ)分析擴(kuò)增效果。結(jié)果表明，使用REA作為抽提試劑提取的RNA對下游qPCR實(shí)驗(yàn)無擴(kuò)增抑制。
DNA殘留量低
使用TransGen ER501產(chǎn)品，分別以氯仿和REA作為抽提試劑 ，以提取的Hela細(xì)胞RNA為模板，人gDNA為引物，用染料法qPCR產(chǎn)品(AQ601)分析DNA殘留量。結(jié)果表明，使用REA作為抽提試劑提取的RNA中DNA殘留量低。
適用多物種RNA的提取
使用TransGen ER501產(chǎn)品，分別以REA和氯仿作為抽提試劑，提取293T細(xì)胞、小鼠肝組織、煙草的RNA，瓊脂糖凝膠電泳分析提取效果。結(jié)果表明，TransGen產(chǎn)品可用于提取多物種RNA。
1 Ye X, Xu L, Li X, et al. miR-34 modulates wing polyphenism in planthopper[J]. PLoS Genetics, 2019.(IF 5.22)
2 Zhao Z, Bao X, Zhang Z, et al. Novel phloroglucinol derivative Compound 21 protects experimental autoimmune encephalomyelitis rats via inhibiting Th1/Th17 cell infiltration[J]. Brain, Behavior, and Immunity, 2020.(IF 6.17)
3 Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)
4 Zhang Y, Liu J, Liu J L. The atlas of cytoophidia in Drosophila larvae[J]. Journal of genetics and genomics, 2020.(IF 5.06)
5 Tan Y, Yan X, Sun J, et al. Genome‐wide enhancer identification by massively parallel reporter assay in Arabidopsis[J]. The Plant Journal, 2023.(IF 7.20)
6 Wang B, Tang X, Yao L, et al. Disruption of USP9X in macrophages promotes foam cell formation and atherosclerosis[J]. The Journal of Clinical Investigation, 2022.(IF 14.80)
7 Li X, Zhou L, Gao B Q, et al. Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure[J]. Nature Communications, 2022.(IF 17.69)
8 Du P, Li N, Xiong X, et al. A bivalent vaccine containing D614G and BA. 1 spike trimer proteins or a BA. 1 spike trimer protein booster shows broad neutralizing immunity[J]. Journal of Medical Virology, 2022.(IF 20.69)
9 Guo Z, Cao H, Zhao J, et al. A natural uORF variant confers phosphorus acquisition diversity in soybean[J]. Nature Communications, 2022.(IF 17.69)
10 Zhao Z, Ning J, Bao X, et al. Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis[J]. Microbiome, 2021.(IF 16.83)
11 Zhang M, Li F D, Li K, et al. Functional characterization and structural basis of an efficient di-C-glycosyltransferase from Glycyrrhiza glabra[J]. Journal of the American Chemical Society, 2020.(IF 14.61)
12 Wang L, Xue W, Zhang H, et al. Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations[J]. Nature Cell Biology, 2021.(IF 20.04)
