產(chǎn)品詳情介紹
螢火蟲熒光素酶 (firefly luciferase)和海腎螢光素酶(Renilla luciferase)可分別催化熒光素 (luciferin)或腔腸素 (coelenterazine) 氧化形成oxyluciferin或coelenteramide ，并在此過程中產(chǎn)生生物熒光。TransDetect? Double-Luciferase Reporter Assay Kit首先以熒光素為底物檢測螢火蟲熒光素酶報告基因的活性，之后在淬滅該熒光反應(yīng)的同時，以腔腸素為底物檢測海腎熒光素酶報告基因的活性，具有檢測迅速、靈敏度高、檢測范圍廣、無細(xì)胞內(nèi)源活性干擾等特點。
1 Ji X, Du Y, Li F, et al. The basic helix‐loop‐helix transcription factor, Os PIL 15, regulates grain size via directly targeting a purine permease gene Os PUP 7 in rice[J]. Plant Biotechnology Journal, 2019.(IF 6.30)
2 Li Y, Feng D, Wang Z, et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion[J]. Cell Death & Differentiation, 2019.(IF 8.00)
3 Liu F, Xu Y, Chang K, et al. The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis[J]. New Phytologist, 2019.(IF 7.43)
4 Qi Y, Ding L, Zhang S, et al. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency[J]. Cell, 2022.(IF 41.58)
5 Cheng Q, Wang J, Li M, et al. CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease[J]. Redox Biology, 2022.(IF 10.78)
6 Di Y, Jing X, Hu K, et al. The c-MYC-WDR43 signalling axis promotes chemoresistance and tumour growth in colorectal cancer by inhibiting p53 activity[J]. Drug Resistance Updates, 2023.(IF 22.84)
7 Jia X, Wang L, Zhao H, et al. The origin and evolution of salicylic acid signaling and biosynthesis in plants[J]. Molecular Plant, 2023.(IF 21.94)
8 Liu Y, Zhang S, Chen L, et al. The molecular mechanism of miR-96-5p in the pathogenesis and treatment of polycystic ovary syndrome[J]. Translational Research, 2023.(IF 10.17)
9 Zhou J, Ge Q, Wang D, et al. Engineering a modular double-transmembrane synthetic receptor system for customizing cellular programs[J]. Cell Reports, 2023.(IF 9.99)
10 Cui Y, Lyu X, Ding L, et al. Global miRNA dosage control of embryonic germ layer specification[J]. Nature, 2021.(IF 42.00)
